Sequencing services

Single-cell immune repertoire profiling

Paired 5' transcriptome and V(D)J from the same cell, for animal models.

01What it is

Work runs at Beijing BestopCell Co., Ltd. on their CelCode® platform. Cells are encapsulated with barcoded gel beads, reverse-transcribed in droplet, and the resulting full-length cDNA is split: one part builds the 5' transcriptome library, the other builds BCR and/or TCR libraries by nested V(D)J amplification. Both come from the same barcode, so clonotype and expression phenotype stay linked at the single-cell level.

Full-length cDNAone barcode per cell
5′ transcriptomefragment, adapter, index
BCR / TCRnested V(D)J amplification
Clonotype + phenotypelinked at the single cell

Both libraries carry the same cell barcode, which is what keeps a receptor sequence attached to the expression state of the cell it came from.

02Specifications

Numbers, not adjectives.

Cells recovered	Up to 20,000 per sample
Cell capture rate	Up to 65%
Samples per run	1–4, loaded flexibly
Encapsulation time	6 minutes
Cells to library	8 hours
Sequencing mode	PE150, dual 10 bp index
Read structure	R1 150 bp barcode + UMI · R2 150 bp mRNA / V(D)J insert
Platforms	Illumina NovaSeq 6000 · NovaSeq X Plus · MGI DNBSEQ-T7 · GeneMind SURFSeq 5000

03Output

What the output looks like.

Example V(D)J output from the platform — not results from your samples, and not ours. This is the shape of what comes back.

Bar chart of clonotype frequency, with one dominant clonotype and a long tail — Clonotype frequency. Each bar is one clonotype; height is the number of cell barcodes carrying it.

Bar chart of barcode frequency across TRA and TRB V genes — V gene usage across TRA and TRB.

Heatmap of VJ combination frequencies across TRA and TRB V genes — VJ combination frequencies.

Scatter plot of paired clonotype diversity over V and D gene combinations — Paired clonotype diversity over V and D gene combinations.

Included on the transcriptome side.

The 5′ library is analysed as well as sequenced. These run as standard.

Bar chart of enriched pathways beside a GSEA enrichment plot — GO / KEGG enrichment, with GSEA.

Protein-protein interaction network with two coloured modules — Protein interaction network.

Network of predicted transcription factors and their target genes — Transcription-factor prediction.

Cells projected in two dimensions and coloured by inferred cell-cycle phase — Cell-cycle phase per cell.

Inferred copy-number heatmap across chromosomes for each cell cluster — CNV inference across chromosomes.

Figures supplied by BestopCell.

04Species

V(D)J kits available.

The 5' transcriptome side works for any species with polyadenylated mRNA. Paired V(D)J amplification needs a species-matched kit — these exist today:

Mouse — TCR and BCR
Rabbit — TCR and BCR
Monkey — TCR and BCR
Human — TCR and BCR (see limits)

05Deliverables

Also included as standard.

Cell clustering and cell-type annotation
Multi-sample integration
Differential expression with clustering heatmap
Pseudotime trajectory
Ligand–receptor and cell communication
Per-sample metrics summary

06Cost

What it costs.

Kit content, at manufacturer list price

Kit	Pack	List	Per reaction
5′ single-cell transcriptome kit set	16 rxn	USD 23,810	USD 1,490 / rxn
TCR amplification kit (per species)	16 rxn	USD 595	USD 35 / rxn
BCR amplification kit (per species)	16 rxn	USD 595	USD 35 / rxn
Immune repertoire library kit	16 rxn	USD 1,190	USD 75 / rxn
Tissue dissociation kit (most tissues)	10 rxn	USD 595	USD 60 / rxn

Kits are sold in whole packs, so a study is costed by pack rather than by sample.

A 16-sample study, 5′ transcriptome with paired TCR and BCR

5′ single-cell transcriptome kit set	USD 23,810
TCR amplification kit	USD 595
BCR amplification kit	USD 595
Immune repertoire library kit	USD 1,190
2 × Tissue dissociation kit (10 rxn)	USD 1,190
Total, 16 samples	USD 27,380
Per sample	USD 1,710

Sequencing, freight, duty, and dry-ice handling are quoted separately.

Manufacturer list prices, converted from CNY. A 16-reaction kit covers 16 samples; the per-sample column is what one sample consumes. Converted at 0.1488 USD/CNY, 2026-08-23.

07Limits

What bounds this.

Animal models first. Human-sample projects require ethics review — an REB in Canada, an IRB in the United States — covering export, a material transfer agreement, institutional data-residency policy, and China's human genetic resources regulations on the receiving end. Each is solvable; none is quick.
Research use only.
Raw sequence data is not assumed. The supplier's own material describes delivery as a report; if you need FASTQ, we get that written into the order before anything ships.
One pilot shipment runs end to end before anything is proposed at scale.

08Next

Worth a conversation?

Tell us the species, the sample type, roughly how many, and when. Get in touch.